Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca2+ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea-Reference-Cited by-同舟云学术

Organ-on-chip model shows that ATP release through connexin hemichannels drives spontaneous Ca2+ signaling in non-sensory cells of the greater epithelial ridge in the developing cochlea

Published:2020 Issue:16 Volume:20 Page:3011-3023
ISSN:1473-0197
Container-title:Lab on a Chip
language:en
Short-container-title:Lab Chip

Author:

Mazzarda Flavia¹²³⁴⁵^ORCID,D'Elia Annunziata¹²³⁴⁵^ORCID,Massari Roberto¹²³^ORCID,De Ninno Adele⁶²³^ORCID,Bertani Francesca Romana⁶²³^ORCID,Businaro Luca⁶²³^ORCID,Ziraldo Gaia¹²³⁷⁸^ORCID,Zorzi Veronica¹²³⁷⁸^ORCID,Nardin Chiara¹²³^ORCID,Peres Chiara¹²³^ORCID,Chiani Francesco¹²³^ORCID,Tettey-Matey Abraham¹²³^ORCID,Raspa Marcello¹²³^ORCID,Scavizzi Ferdinando¹²³^ORCID,Soluri Alessandro¹²³^ORCID,Salvatore Anna Maria¹²³^ORCID,Yang Jun⁹¹⁰¹¹¹²¹³^ORCID,Mammano Fabio¹²³¹⁴¹⁵^ORCID

Affiliation:

1. CNR Institute of Biochemistry and Cell Biology

2. Rome

3. Italy

4. Department of Science

5. Università degli Studi di Roma3

6. CNR Institute for Photonics and Nanotechnology

7. Institute of Otorhinolaryngology

8. Università Cattolica del Sacro Cuore

9. Department of Otorhinolaryngology Head and Neck Surgery

10. Xinhua Hospital

11. Shanghai Jiao Tong University School of Medicine

12. Shanghai

13. China

14. Department of Physics and Astronomy “G. Galilei”

15. University of Padova

Abstract

Using microfluidics, ATP biosensors, multiphoton microscopy and genetically targeted mice, we show that ATP release through connexin hemichannels, and not pannexin 1 channels, underlies spontaneous Ca²⁺ wave propagation in the greater epithelial ridge of the developing cochlea.

Funder

Università degli Studi di Padova

Consiglio Nazionale delle Ricerche

Fondazione Telethon

Ministero dell’Istruzione, dell’Università e della Ricerca

Publisher

Royal Society of Chemistry (RSC)

Subject

Biomedical Engineering,General Chemistry,Biochemistry,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/LC/D0LC00427H

Reference93 articles.