Unleashing the potential: type I CRISPR-Cas systems in actinomycetes for genome editing

Author:

Wang Shuliu1,Zeng Xiaoqian1,Jiang Yue1,Wang Weishan2ORCID,Bai Linquan3,Lu Yinhua4,Zhang Lixin1,Tan Gao-Yi1ORCID

Affiliation:

1. State Key Laboratory of Bioreactor Engineering (SKLBE), School of Biotechnology, East China University of Science and Technology (ECUST), Shanghai 200237, China

2. State Key Laboratory of Microbial Resources and CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Beijing 100101, China

3. State Key Laboratory of Microbial Metabolism, School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China

4. College of Life Sciences, Shanghai Normal University, Shanghai 200234, China

Abstract

This review introduces the potential of type I CRISPR-Cas systems in actinomycetes for genome editing and discusses how to establish and develop genome editing tools based on type I CRISPR-Cas systems in actinomycetes.

Funder

National Natural Science Foundation of China

National Key Research and Development Program of China

Higher Education Discipline Innovation Project

Fundamental Research Funds for the Central Universities

Publisher

Royal Society of Chemistry (RSC)

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