Complete enzymatic digestion of double-stranded RNA to nucleosides enables accurate quantification of dsRNA-Reference-Cited by-同舟云学术

Complete enzymatic digestion of double-stranded RNA to nucleosides enables accurate quantification of dsRNA

Published:2021 Issue:2 Volume:13 Page:179-185
ISSN:1759-9660
Container-title:Analytical Methods
language:en
Short-container-title:Anal. Methods

Author:

Strezsak Steven R.¹²³⁴⁵^ORCID,Beuning Penny J.¹²³⁴,Skizim Nicholas J.⁵⁶⁴

Affiliation:

1. Department of Chemistry & Chemical Biology

2. Northeastern University

3. Boston

4. USA

5. Greenlight Biosciences

6. Medford

Abstract

The rapid growth of research focusing on RNA, especially for RNA interference applications, has created a need for a robust method that can accurately determine the concentration of long dsRNA.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Chemical Engineering,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2021/AY/D0AY01498B

Reference16 articles.

1. Introduction to micro-analytical systems: bioanalytical and pharmaceutical applications

2. Revised UV extinction coefficients for nucleoside-5′-monophosphates and unpaired DNA and RNA

3. Fragmentation of DNA by Sonication: Figure 1.

4. Base-stacking and base-pairing contributions into thermal stability of the DNA double helix

5. RNASwift: A rapid, versatile RNA extraction method free from phenol and chloroform

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