New insights into the oxidation process from neutron and X-ray crystal structures of an O<sub>2</sub>-sensitive [NiFe]-hydrogenase-Reference-Cited by-同舟云学术

New insights into the oxidation process from neutron and X-ray crystal structures of an O₂-sensitive [NiFe]-hydrogenase

Published:2023 Issue:35 Volume:14 Page:9306-9315
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Hiromoto Takeshi¹²,Nishikawa Koji²,Inoue Seiya²,Ogata Hideaki²^ORCID,Hori Yuta³^ORCID,Kusaka Katsuhiro⁴,Hirano Yu¹⁵,Kurihara Kazuo¹,Shigeta Yasuteru¹³^ORCID,Tamada Taro¹⁵^ORCID,Higuchi Yoshiki²^ORCID

Affiliation:

1. Institute for Quantum Life Science, National Institutes for Quantum Science and Technology, 4-9-1 Anagawa, Inage, Chiba 263-8555, Japan

2. Graduate School of Science, University of Hyogo, 3-2-1 Koto, Kamigori, Hyogo 678-1297, Japan

3. Center for Computational Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan

4. Neutron Industrial Application Promotion Center, Comprehensive Research Organization for Science and Society, 162-1 Shirakata, Tokai, Ibaraki 319-1106, Japan

5. Department of Quantum Life Science, Graduate School of Science, Chiba University, 1-33 Yayoi, Inage, Chiba 263-8522, Japan

Abstract

We report the first neutron structure of [NiFe]-hydrogenase in its oxidized state. This study leads to new insights into the oxidized active site and visualization of the protons characteristic of the oxidized enzyme.

Funder

Japan Science and Technology Corporation

Ministry of Education, Culture, Sports, Science and Technology

Japan Society for the Promotion of Science

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/SC/D3SC02156D

Reference55 articles.

1. Occurrence, Classification, and Biological Function of Hydrogenases: An Overview