A stationary phase with a positively charged surface allows for minimizing formic acid concentration in the mobile phase, enhancing electrospray ionization in LC-MS proteomic experiments-Reference-Cited by-同舟云学术

A stationary phase with a positively charged surface allows for minimizing formic acid concentration in the mobile phase, enhancing electrospray ionization in LC-MS proteomic experiments

Published:2023 Issue:23 Volume:148 Page:5980-5990
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Jadeja Siddharth¹,Kupcik Rudolf²,Fabrik Ivo²,Sklenářová Hana¹^ORCID,Lenčo Juraj¹^ORCID

Affiliation:

1. Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Heyrovského 1203/8, 500 03 Hradec Králové, Czech Republic

2. Biomedical Research Centre, University Hospital Hradec Králové, Sokolská 581, 500 05 Hradec Králové, Czech Republic

Abstract

If a column packed with a stationary phase with a positively charged surface is used for proteomic LC-MS experiments, the concentration of formic acid in the mobile phase can be decreased to 0.01%, significantly increasing the MS sensitivity.

Funder

Grantová Agentura České Republiky

Grantová Agentura, Univerzita Karlova

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2023/AN/D3AN01508D

Reference49 articles.

1. Protein Analysis by Shotgun/Bottom-up Proteomics

2. Proteomics: a pragmatic perspective