Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification-Reference-Cited by-同舟云学术

Highly sensitive and multiplexed quantification of mRNA splice variants by the direct ligation of DNA probes at the exon junction and universal PCR amplification

Published:2017 Issue:5 Volume:8 Page:3635-3640
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Wang Honghong¹²³⁴⁵^ORCID,Wang Hui¹²³⁴⁵^ORCID,Duan Xinrui¹²³⁴⁵,Sun Yuanyuan¹²³⁴⁵,Wang Xiangdong¹²³⁴⁵,Li Zhengping¹²³⁴⁵^ORCID

Affiliation:

1. Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province

2. School of Chemistry and Chemical Engineering

3. Shaanxi Normal University

4. Xi’an 710062

5. P. R. China

Abstract

A highly sensitive and specific assay for detecting mRNA splice variants is developed based on ligation-dependent PCR.

Funder

National Natural Science Foundation of China

Shaanxi Normal University

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2017/SC/C7SC00094D

Reference30 articles.

1. Alternative Splicing: New Insights from Global Analyses

2. Alternative splicing in disease and therapy

3. Epigenetics in Alternative Pre-mRNA Splicing

4. Genome-wide detection of tissue-specific alternative splicing in the human transcriptome

5. Revealing Global Regulatory Features of Mammalian Alternative Splicing Using a Quantitative Microarray Platform

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