On the role of citrate in 12-molybdophosphoric-acid methods for quantification of phosphate in the presence of ATP-Reference-Cited by-同舟云学术

On the role of citrate in 12-molybdophosphoric-acid methods for quantification of phosphate in the presence of ATP

Published:2022 Issue:25 Volume:46 Page:12401-12409
ISSN:1144-0546
Container-title:New Journal of Chemistry
language:en
Short-container-title:New J. Chem.

Author:

Recoulat Angelini Alvaro A.¹^ORCID,Martínez Gache Santiago A.¹,Sabeckis M. Lurdes¹,Melian Noelia A.¹,González Flecha F. Luis¹^ORCID

Affiliation:

1. Universidad de Buenos Aires-CONICET, Laboratorio de Biofísica Molecular, Instituto de Química y Fisicoquímica Biológicas, Buenos Aires, Argentina

Abstract

Citrate is a useful additive for measuring ATPase activities avoiding the interference of phosphate release after catalysis. In this work we explore this effect, and optimize the malachite green procedure for determination of phosphate.

Funder

Consejo Nacional de Investigaciones Científicas y Técnicas

Fondo para la Investigación Científica y Tecnológica

Universidad de Buenos Aires

Publisher

Royal Society of Chemistry (RSC)

Subject

Materials Chemistry,General Chemistry,Catalysis

Link

http://pubs.rsc.org/en/content/articlepdf/2022/NJ/D2NJ00943A

Reference29 articles.

1. Why nature really chose phosphate

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4. The molybdenum blue reaction for the determination of orthophosphate revisited: Opening the black box

5. Determination of phosphate: Study of labile organic phosphate interference

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