One-step methodology for the direct covalent capture of GPCRs from complex matrices onto solid surfaces based on the bioorthogonal reaction between haloalkane dehalogenase and chloroalkanes-Reference-Cited by-同舟云学术

One-step methodology for the direct covalent capture of GPCRs from complex matrices onto solid surfaces based on the bioorthogonal reaction between haloalkane dehalogenase and chloroalkanes

Published:2018 Issue:2 Volume:9 Page:446-456
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Zeng Kaizhu¹²³⁴⁵,Li Qian¹²³⁴⁵,Wang Jing¹²³⁴⁵,Yin Guowei⁶⁷⁸^ORCID,Zhang Yajun¹²³⁴⁵,Xiao Chaoni¹²³⁴⁵,Fan Taiping¹²³⁴⁵,Zhao Xinfeng¹²³⁴⁵^ORCID,Zheng Xiaohui¹²³⁴⁵

Affiliation:

1. Key Laboratory of Resource Biology and Biotechnology in Western China

2. Ministry of Education

3. College of Life Sciences

4. Northwest University

5. Xi’an 710069

6. Department of Biochemistry and Biophysics

7. University of North Carolina at Chapel Hill

8. USA

Abstract

An approach is established for the specific immobilization of GPCRs from cell lysates that circumvents labor intensive purification procedures and minimize loss of activity.

Funder

Ministry of Education of the People’s Republic of China

National Natural Science Foundation of China

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2018/SC/C7SC03887A

Reference45 articles.

1. Label-Free Real-Time Microarray Imaging of Cancer Protein–Protein Interactions and Their Inhibition by Small Molecules

2. Detection of Protein–Small Molecule Binding Using a Self-Referencing External Cavity Laser Biosensor