Simultaneous improvement of the thermostability and activity of lactic dehydrogenase from <i>Lactobacillus rossiae</i> through rational design-Reference-Cited by-同舟云学术

Simultaneous improvement of the thermostability and activity of lactic dehydrogenase from Lactobacillus rossiae through rational design

Published:2022 Issue:51 Volume:12 Page:33251-33259
ISSN:2046-2069
Container-title:RSC Advances
language:en
Short-container-title:RSC Adv.

Author:

Luo Xi¹,Wang Yifeng¹²,Zheng Weilong¹,Sun Xiaolong¹,Hu Gaowei¹,Yin Longfei¹,Zhang Yingying¹,Yin Fengwei¹,Fu Yongqian¹^ORCID

Affiliation:

1. Institute of Biomass Resources, Taizhou University, Taizhou 318000, Zhejiang, People's Republic of China

2. School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu 210023, China

Abstract

The d-LDH was engineered using computationally-assisted rational mutagenesis. The two mutants D249A and D249A/T247I showed significantly enhanced thermostability and catalytic activity to sodium phenylpyruvate compared with the wild-type enzyme.

Publisher

Royal Society of Chemistry (RSC)

Subject

General Chemical Engineering,General Chemistry

Link

http://pubs.rsc.org/en/content/articlepdf/2022/RA/D2RA05599F

Reference39 articles.

1. Recent development of phenyllactic acid: physicochemical properties, biotechnological production strategies and applications

2. Bioprotective mechanisms of lactic acid bacteria against fungal spoilage of food

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5. Antibacterial activity of phenyllactic acid against Listeria monocytogenes and Escherichia coli by dual mechanisms

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