Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers-Reference-Cited by-同舟云学术

Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers

Published:2020 Issue:4 Volume:2 Page:1625-1633
ISSN:2516-0230
Container-title:Nanoscale Advances
language:en
Short-container-title:Nanoscale Adv.

Author:

Birchenough Holly L.¹²,Swann Marcus J.³⁴²,Zindy Egor¹²,Day Anthony J.¹²,Jowitt Thomas A.¹²^ORCID

Affiliation:

1. Wellcome Trust Centre for Cell-Matrix Research

2. UK

3. Swann Scientific Consulting Ltd

4. Lymm WA13 9HR

Abstract

We have shown using SEEC microscopy that avidin addition to Biotinyl-Cap-PE lipids forms clusters, reducing the levels of association. By using PDP-PE lipids with PEG linkers, we have achieved much higher avidin levels with no clustering effects.

Funder

Wellcome Trust

Biotechnology and Biological Sciences Research Council

Publisher

Royal Society of Chemistry (RSC)

Subject

General Engineering,General Materials Science,General Chemistry,Atomic and Molecular Physics, and Optics,Bioengineering

Link

http://pubs.rsc.org/en/content/articlepdf/2020/NA/D0NA00060D

Reference37 articles.

1. Controlling Multivalent Binding through Surface Chemistry: Model Study on Streptavidin

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3. Comparative surface antimicrobial properties of synthetic biocides and novel human apolipoprotein E derived antimicrobial peptides

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