Reverse transcription as key step in RNA <i>in vitro</i> evolution with unnatural base pairs-Reference-Cited by-同舟云学术

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Reverse transcription as key step in RNA in vitro evolution with unnatural base pairs

Published:2024 Issue:6 Volume:5 Page:556-566
ISSN:2633-0679
Container-title:RSC Chemical Biology
language:en
Short-container-title:RSC Chem. Biol.

Author:

Hoffmann Eva S.¹,De Pascali Mareike C.²³,Neu Lukas¹,Domnick Christof¹,Soldà Alice³,Kath-Schorr Stephanie¹^ORCID

Affiliation:

1. Institute of Organic Chemistry, Department of Chemistry, University of Cologne, Greinstrasse 4, 50939 Cologne, Germany

2. Technical University of Munich, TUM School of Natural Sciences, Department of Bioscience, Garching, 85748, Germany

3. Dynamic Biosensors GmbH, Perchtinger Str. 8/10, Munich, 81379, Germany

Abstract

Unnatural base pairs (UBPs) augment the chemical diversity of artificial nucleic acids and can enable the generation of new aptamers and catalytic nucleic acids by in vitro selection. Reverse transcription of UBPs as key step during RNA in vitro selection is investigated.

Funder

Boehringer Ingelheim Stiftung

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/CB/D4CB00084F

Reference43 articles.

1. The Expanded Genetic Alphabet

2. Creation of unnatural base pairs for genetic alphabet expansion toward synthetic xenobiology

3. Genetic alphabet expansion technology by creating unnatural base pairs

4. In vitro selection of RNA molecules that bind specific ligands

5. Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA Polymerase

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