Volatile metabolites differentiate air–liquid interface cultures after infection with Staphylococcus aureus

Author:

Ahmed Waqar1ORCID,Bardin Emmanuelle23,Davis Michael D.4,Sermet-Gaudelus Isabelle25,Grassin Delyle Stanislas36ORCID,Fowler Stephen J.17ORCID

Affiliation:

1. Division of Immunology, Immunity to infection & Respiratory Medicine, Faculty of Biology, Medicine, and Health, University of Manchester, Manchester, UK

2. Institut Necker-Enfants Malades, Paris, France

3. Université Paris-Saclay, UVSQ, INSERM, Infection et inflammation, Montigny le Bretonneux, France

4. Herman B Wells Center for Pediatric Research, Pediatric Pulmonology, Allergy, and Sleep Medicine, Indiana University School of Medicine, Indianapolis, USA

5. Service de Pneumo-Pédiatrie, Université René Descartes, Hôpital Necker-Enfants Malades, Paris, France

6. Hôpital Foch, Exhalomics, Département des maladies des voies respiratoires, Suresnes, France

7. NIHR Manchester Biomedical Research Centre, Manchester University Hospitals NHS Foundation Trust, Education and Research Centre, W ythenshawe Hospital, Manchester, M23 9LT, UK

Abstract

Thin film microextraction to sample VOCs from the apical side of an air–liquid interface culture model. After S. aureus infection, infected and uninfected cultures were distinguished using an untargeted metabolomics approach.

Publisher

Royal Society of Chemistry (RSC)

Subject

Electrochemistry,Spectroscopy,Environmental Chemistry,Biochemistry,Analytical Chemistry

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