High-throughput direct screening of restriction endonuclease using a microfluidic fluorescence-activated drop sorter based on the SOS response in <i>Escherichia coli</i>-Reference-Cited by-同舟云学术

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High-throughput direct screening of restriction endonuclease using a microfluidic fluorescence-activated drop sorter based on the SOS response in Escherichia coli

Published:2024 Issue:13 Volume:149 Page:3575-3584
ISSN:0003-2654
Container-title:The Analyst
language:en
Short-container-title:Analyst

Author:

Zhang Yizhe¹²^ORCID,Agresti Jeremy J.²,Zheng Yu³,Weitz David A.²⁴^ORCID

Affiliation:

1. Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA

2. John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts, USA

3. New England BioLabs, Inc., Ipswich, MA 01938, USA

4. Department of Physics, Harvard University, Cambridge, MA 02138, USA

Abstract

The E. coli SOS pathway is utilized for high-throughput screening of restriction enzymes through a microfluidic drop sorter.

Funder

National Science Foundation

Materials Research Science and Engineering Center, Harvard University

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/AN/D4AN00106K

Reference30 articles.

1. A Restriction enzyme from Hemophilus influenzae

2. Production of Specific Fragments of φX174 Replicative Form DNA by a Restriction Enzyme from Haemophilus parainfluenzae , Endonuclease HP

3. High-frequency modification of plant genes using engineered zinc-finger nucleases

4. TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain

5. De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks

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