Crucial gating residues govern the enhancement of peroxygenase activity in an engineered cytochrome P450 <i>O</i>-demethylase-Reference-Cited by-同舟云学术

Crucial gating residues govern the enhancement of peroxygenase activity in an engineered cytochrome P450 O-demethylase

Published:2024 Issue:21 Volume:15 Page:8062-8070
ISSN:2041-6520
Container-title:Chemical Science
language:en
Short-container-title:Chem. Sci.

Author:

Zhao Panxia¹²,Jiang Yiping¹³⁴,Wang Qian¹,Chen Jie¹³⁴,Yao Fuquan¹,Cong Zhiqi¹³²⁴^ORCID

Affiliation:

1. CAS Key Laboratory of Biofuels, Shandong Provincial Key Laboratory of Synthetic Biology Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences Qingdao, Shandong, P. R. China

2. University of Chinese Academy of Sciences (UCAS), Beijing 100049, P. R. China

3. Shandong Energy Institute, Qingdao, Shandong, 266101, China

4. Qingdao New Energy Shandong Laboratory Qingdao, Shandong, P. R. China

Abstract

The structurally crucial gating effect is elucidated for regulating the inflow of H2O2 to enhance the peroxygenase activity of engineered CYP199A4 variants, which showed O-demethylation activity toward a variety of methoxy-substituted benzoic acids.

Funder

National Key Research and Development Program of China

National Natural Science Foundation of China

Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences

Publisher

Royal Society of Chemistry (RSC)

Link

http://pubs.rsc.org/en/content/articlepdf/2024/SC/D4SC02418D

Reference29 articles.

1. Trends in Lignin Biotransformations for Bio-Based Products and Energy Applications

2. Identifying and creating pathways to improve biological lignin valorization

3. Microbial degradation of aromatic compounds — from one strategy to four

4. A Tetrahydrofolate-Dependent O -Demethylase, LigM, Is Crucial for Catabolism of Vanillate and Syringate in Sphingomonas paucimobilis SYK-6

5. A Novel Tetrahydrofolate-Dependent O -Demethylase Gene Is Essential for Growth of Sphingomonas paucimobilis SYK-6 with Syringate