Near-infrared rhodol dyes bearing salicylaldehyde moieties for ratiometric pH sensing in live cells during mitophagy and under hypoxia conditions

Author:

Dwivedi Sushil K.1,Arachchige Dilka Liyana1,Vohs Tara1,Tang Jiani2,Usimaki Kyle1,Olowolagba Adenike Mary1ORCID,Fritz Delaney Raine3,Luck Rudy L1ORCID,Werner Thomas3,Liu Haiying1ORCID

Affiliation:

1. Department of Chemistry, and Research Health Institute, Michigan Technological University, Houghton, MI 49931, USA

2. High School, Houghton Portage Township Schools, Houghton, MI 49931, USA

3. Department of Biological Sciences, Michigan Technological University, Houghton, MI 49931, USA

Abstract

We describe a simple but efficient approach to make fluorescent probes A and B based on rhodol dyes incorporated with salicyaldehyde moiety for monitoring pH changes in mitochondria under oxidative stresses and hypoxia conditions, and for tracking mitophagy processes.

Funder

National Science Foundation

National Institutes of Health

Publisher

Royal Society of Chemistry (RSC)

Subject

General Materials Science,Biomedical Engineering,General Chemistry,General Medicine

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