Geometry and length control of 3D engineered heart tissues using direct laser writing

Author:

Karakan M. Çağatay1234ORCID,Ewoldt Jourdan K.34ORCID,Segarra Addianette J.25,Sundaram Subramanian34ORCID,Wang Miranda C.346ORCID,White Alice E.12378ORCID,Chen Christopher S.34,Ekinci Kamil L.127ORCID

Affiliation:

1. Department of Mechanical Engineering, Boston University, Boston, MA 02215, USA

2. Photonics Center, Boston University, Boston, MA 02215, USA

3. Department of Biomedical Engineering, Boston University, Boston, MA 02215, USA

4. Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA

5. Department of Biomedical Engineering, Polytechnic University of Puerto Rico, San Juan 00918, Puerto Rico

6. Harvard-MIT Program in Health Sciences and Technology, Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA

7. Division of Materials Science and Engineering, Boston University, Boston, Massachusetts 02215, USA

8. Department of Physics, Boston University, Boston, MA 02215, USA

Abstract

Using two-photon direct laser writing, we developed a versatile platform to generate, scale, and study hiPSC-derived engineered heart tissues (EHTs) in various geometries, with the goal of promoting fiber alignment and maturation of the EHTs.

Funder

National Science Foundation

National Institutes of Health

NIH Office of the Director

Publisher

Royal Society of Chemistry (RSC)

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