Affiliation:
1. Laboratory of Animal Biotechnology, Federal Rural University of Semi-Arid, Mossoro, RN, Brazil
Abstract
BACKGROUND: The synergistic action among the different extracellular cryoprotectants could improve somatic cell quality after thawing and provide bases for the formation of biobanks for redrumped agoutis. OBJECTIVE: This study evaluated the interactions among sucrose (SUC)
and concentrations of serum fetal bovine (FBS) on the cryopreservation of somatic cells derived from redrumped agoutis. MATERIALS AND METHODS: Cells were cryopreserved with 10% dimethyl sulfoxide and different concentrations of FBS (10%, 40%, and 90%) with or without 0.2 M SUC, totaling
six comparison groups. Non-cryopreserved cells were used as a control. Cells were evaluated for viability, metabolic activity, proliferative activity, reactive oxygen species (ROS), mitochondrial membrane potential (ΔΨm) and apoptosis levels. RESULTS: No difference was observed
among cryopreserved with DMSO containing (10FBS, 10FBS-SUC, 40FBS, 40FBS-SUC, 90FBS, 90FBSSUC) and non-cryopreserved groups for viability, metabolic activity, proliferative activity, and ROS levels. Interestingly, only cells cryopreserved with 90% FBS and SUC maintained the ΔΨm like
the control. This indicates that at high concentrations of FBS, SUC contributes to the maintenance of this parameter in cryopreserved cells. Moreover, at concentrations of 10% and 40% of FBS, SUC contributed to the maintenance of viability evaluated by the levels of apoptosis evaluated after
thawing. In summary, we verified that 90% FBS and 0.2 M SUC promote greater ability of cells after thawing. Additionally, SUC positively acts in cryopreservation solutions containing 10% and 40% FBS. CONCLUSION: This information is essential to an understanding of the mechanisms involved
in the interactions of extracellular cryoprotectants in somatic cell cryopreservation solutions of red-rumped agoutis.
Publisher
CryoLetters Limited Liability Partnership
Cited by
1 articles.
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