Author:
Veselkov Dennis A.,Laponogov Ivan,Pan Xiao-Su,Selvarajah Jogitha,Skamrova Galyna B.,Branstrom Arthur,Narasimhan Jana,Prasad Josyula V. N. Vara,Fisher L. Mark,Sanderson Mark R.
Abstract
Klebsiella pneumoniaeis a Gram-negative bacterium that is responsible for a range of common infections, including pulmonary pneumonia, bloodstream infections and meningitis. Certain strains ofKlebsiellahave become highly resistant to antibiotics. Despite the vast amount of research carried out on this class of bacteria, the molecular structure of its topoisomerase IV, a type II topoisomerase essential for catalysing chromosomal segregation, had remained unknown. In this paper, the structure of its DNA-cleavage complex is reported at 3.35 Å resolution. The complex is comprised of ParC breakage-reunion and ParE TOPRIM domains ofK. pneumoniaetopoisomerase IV with DNA stabilized by levofloxacin, a broad-spectrum fluoroquinolone antimicrobial agent. This complex is compared with a similar complex fromStreptococcus pneumoniae, which has recently been solved.
Publisher
International Union of Crystallography (IUCr)
Cited by
34 articles.
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