Abstract
Nanobodies (VHHs) are single-domain antibodies with three antigenic CDR regions and are used in diverse scientific applications. Here, an ∼14 kDa nanobody (A5) specific for the endonuclease VIII (Nei)-like 1 or NEIL1 DNA glycosylase involved in the first step of the base-excision repair pathway was crystallized and its structure was determined to 2.1 Å resolution. The crystals posed challenges due to potential twinning and anisotropic diffraction. Despite inconclusive twinning indicators, reprocessing in an orthorhombic setting and molecular replacement in space group P21212 enabled the successful modeling of 96% of residues in the asymmetric unit, with final R
work and R
free values of 0.199 and 0.229, respectively.
Funder
Basic Energy Sciences
National Institutes of Health, National Institute of Environmental Health Sciences
Bundesministerium für Bildung und Forschung
Deutsche Forschungsgemeinschaft
University of South Alabama
Publisher
International Union of Crystallography (IUCr)