Crystallization and preliminary X-ray study of chloroplast glyceraldehyde-3-phosphate dehydrogenase

Abstract

Glyceraldehyde-3-phosphate dehydrogenase from spinach chloroplasts has been crystallized by vapour diffusion in the pH range 7–8.5 in (NH4)2SO4 and Tris–HCl buffer or potassium phosphate buffer at room temperature. Crystals of the A4 isoform, grown at pH 8.5 in Tris–HCl buffer, diffract to 3.0 Å (at 100 K) using synchrotron radiation. The crystals belong to the orthorhombic C222 space group, with unit-cell dimensions a = 145.9, b = 185.9 and c = 106.3 Å, and probably contain one tetramer per asymmetric unit. Structure determination by molecular replacement is in progress.