Oxidative modification of proteins in rat serum under experimental osteoarthrosis and long-term administration of a multiprobiotic

Abstract

The aim of the study was to investigate the effect of multiprobiotics on the content of products of oxidative modification of proteins and the level of sulfhydryl groups in blood serum of rats during monoiodoacetate-induced osteoarthritis. The study was carried out on white non-linear, sexually mature male rats (weight 180-240 g), according to general ethical principles of experiments on animals. All animals were divided into four experimental groups. The first group - Control: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The second group - Multiprobiotic: animals got injection into knee ligament 0.05 ml of 0.9% NaCl solution on the first day of the experiment and then got intragastric administration 140 mg/kg of multiprobiotic Symbiter® (Prolisok ", Ukraine) diluted in 1 ml of drinking water per 1 kg of animal weight. The third group, MIA-induced OA: animals got injection into knee ligament 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9% NaCl on the first day of the experiment and then got intragastric administration 1 ml of drinking water per 1 kg of the animal weight daily for 14 days from the 8th to 22nd days. The fourth group – MIA-induced OA + Multiprobiotic: animals got injection into knee ligament 0.05 ml of 1 mg of sodium monoiodacetate, dissolved in 0.05 ml of 0.9 % NaCl on the first day of the experiment and then got intragastric administration 140 mg/kg of multiprobiotic diluted in 1 ml of drinking water per 1 kg of animal weight. All animals were killed on day 30 of the experiment, according to the protocol of the ethics committee with rapid blood sampling. The content of the products of oxidative modification of proteins (OMP) and oligopeptides was determined by the level of carbonyl derivatives that were detected in reaction with 2,4-initrophenylhydrazine. The level of total, protein-bound and non-protein sulfhydryl (SH) -groups was measured by the Elman method. It has been established that MIA-induced OA disturbed oxidative-antioxidant balance of the rat serum: the content of the products of oxidative modification of proteins increases and the content of sulfhydryl groups decreases in the serum. It was shown that with the long-term administration of multiprobiotics in animals with MIA-induced OA, the above indicators were restored.