High Quality of Bacterial DNA Extraction from Corbicula fluminea Tissue in Kelantan

Abstract

Corbicula fluminea served as a popular traditional food in Kelantan, Malaysia. This clam is highly consumed by the local people from the last decade. However, there is lack of the study that obtain high quality of bacterial metagenome deoxyribonucleic acid (DNA) extraction from C. fluminea. Improper DNA extraction protocol always fails to extract a high integrity of bacterial DNA bands. This study compares the effectiveness and efficiency of conventional cetyltrimethylammonium bromide (CTAB) protocol, a commercial kit and modified CTAB protocol for bacterial DNA extraction from the soft tissue surface of raw C. fluminea. The instruments used to examine the quality of the extracted bacterial DNA were DeNovix DS-11 spectrophotometer, gel electrophoresis machine and UV transilluminator. The results showed that the bacterial DNA extracted from modified CTAB protocol the highest purity and integrity with the A₂₆₀/A₂₈₀ ratio of 1.92 ± 0.01 as well as the DNA band with minimum smear. This conclude that modified CTAB protocol is the best among the three extraction protocols for the bacterial extraction from the C. fluminea.