Effect of Extraction Time on Degradation of Bioactive Compounds (Zingiber Officinale Roscoe)

Abstract

Ginger, the rhizome of Zingiber officinale contains the bioactive compounds that have a long history of medicinal usage. The two most abundant ginger bioactives identified are 6-gingerol and 6-shogaol. It is important that the availability of these bioactives are first identified. This is done through ethanol extraction. Thus, this paper identified the effect of extraction time using ethanol on the overall yield and concentration of 6-gingerol and 6-shogaol. Kinetic studies on degradation of the bioactive compounds also have been studied. Fourier transform infrared (FTIR) spectroscopy was applied as an additional tool for the characterization of the compounds and in identifying the degradation circumstances. Results showed that the highest overall yield was obtained after 8 hours of extraction. In consistent with the overall yield of extraction, the concentration of 6-gingerol and 6-shogaol reached equilibrium within 8 hours. The concentration of 6-gingerol and 6-shogaol are 13923.26 and 4816.84 μg/g, respectively. Conclusively, the saturated concentration of zingiber bioactive compounds could be determined through ethanol extraction within 8 hours.