Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I-Reference-Cited by-同舟云学术

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Virus and prokaryote enumeration from planktonic aquatic environments by epifluorescence microscopy with SYBR Green I

Published:2007-02 Issue:2 Volume:2 Page:269-276
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Patel Anand,Noble Rachel T,Steele Joshua A,Schwalbach Michael S,Hewson Ian,Fuhrman Jed A

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2007.6.pdf

Reference20 articles.

1. Fuhrman, J.A. Marine viruses and their biogeochemical and ecological effects. Nature 399, 541–548 (1999).

2. Hara, S., Terauchi, K. & Koike, I. Abundance of viruses in marine waters: assessment by epifluorescence and transmission electron microscopy. Appl. Environ. Microbiol. 57, 2731–2734 (1991).

3. Noble, R.T. & Fuhrman, J.A. Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria. Aquatic Microb. Ecol. 14, 113–118 (1998).

4. Weinbauer, M.G. & Suttle, C.A. Comparison of epifluorescence and transmission electron microscopy for counting viruses in natural marine waters. Aquatic Microb. Ecol. 13, 225–232 (1997).

5. Hobbie, J.E., Daley, R.J. & Jasper, S. Use of nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33, 1225–1228 (1977).

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