FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry-Reference-Cited by-同舟云学术

FISH-Flow, a protocol for the concurrent detection of mRNA and protein in single cells using fluorescence in situ hybridization and flow cytometry

Published:2017-05-18 Issue:6 Volume:12 Page:1245-1260
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Arrigucci Riccardo,Bushkin Yuri,Radford Felix,Lakehal Karim,Vir Pooja,Pine Richard,Martin December,Sugarman Jeffrey,Zhao Yanlin,Yap George S,Lardizabal Alfred A,Tyagi Sanjay,Gennaro Maria Laura

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.nature.com/articles/nprot.2017.039.pdf

Reference22 articles.

1. Sanchez, A. & Golding, I. Genetic determinants and cellular constraints in noisy gene expression. Science 342, 1188–1193 (2013).

2. Femino, A.M., Fay, F.S., Fogarty, K. & Singer, R.H. Visualization of single RNA transcripts in situ. Science 280, 585–590 (1998).

3. Raj, A., van den Bogaard, P., Rifkin, S.A., van Oudenaarden, A. & Tyagi, S. Imaging individual mRNA molecules using multiple singly labeled probes. Nat. Methods 5, 877–879 (2008).

4. Rahman, S. & Zenklusen, D. Single-molecule resolution fluorescent in situ hybridization (smFISH) in the yeast S. cerevisiae. Methods Mol. Biol. 1042, 33–46 (2013).

5. Batish, M., Raj, A. & Tyagi, S. Single molecule imaging of RNA in situ. Methods Mol. Biol. 714, 3–13 (2011).

Cited by 74 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes;Nature Communications;2024-09-03

2. Overcoming fixation and permeabilization challenges in flow cytometry by optical barcoding and multi-pass acquisition;2024-08-16

3. Multicolour and lineage-specific interphase chromosome Flow-FISH: method development and clinical validation;Pathology;2024-08

4. FIND-seq: high-throughput nucleic acid cytometry for rare single-cell transcriptomics;Nature Protocols;2024-07-22

5. Shedding Light on Intracellular Proteins using Flow Cytometry;Cell Biochemistry and Biophysics;2024-06-03