Site-specific protein labeling using PRIME and chelation-assisted click chemistry-Reference-Cited by-同舟云学术

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Site-specific protein labeling using PRIME and chelation-assisted click chemistry

Published:2013-07-25 Issue:8 Volume:8 Page:1620-1634
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Uttamapinant Chayasith,Sanchez Mateo I,Liu Daniel S,Yao Jennifer Z,White Katharine A,Grecian Scott,Clark Scott,Gee Kyle R,Ting Alice Y

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2013.096.pdf

Reference31 articles.

1. Uttamapinant, C. et al. Fast, cell-compatible click chemistry with copper-chelating azides for biomolecular labeling. Angew. Chem. Int. Ed. Engl. 51, 5852–5856 (2012).

2. Uttamapinant, C. et al. A fluorophore ligase for site-specific protein labeling inside living cells. Proc. Natl. Acad. Sci. USA 107, 10914–10919 (2010).

3. Liu, D.S. et al. Diels-Alder cycloaddition for fluorophore targeting to specific proteins inside living cells. J. Am. Chem. Soc. 134, 792–795 (2012).

4. Cohen, J.D., Thompson, S. & Ting, A.Y. Structure-guided engineering of a Pacific Blue fluorophore ligase for specific protein imaging in living cells. Biochemistry 50, 8221–8225 (2011).

5. Jin, X., Uttamapinant, C. & Ting, A.Y. Synthesis of 7-aminocoumarin by Buchwald-Hartwig cross coupling for specific protein labeling in living cells. Chembiochem 12, 65–70 (2011).

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