A general protocol for the generation of Nanobodies for structural biology-Reference-Cited by-同舟云学术

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A general protocol for the generation of Nanobodies for structural biology

Published:2014-02-27 Issue:3 Volume:9 Page:674-693
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Pardon Els,Laeremans Toon,Triest Sarah,Rasmussen Søren G F,Wohlkönig Alexandre,Ruf Armin,Muyldermans Serge,Hol Wim G J,Kobilka Brian K,Steyaert Jan

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

https://www.nature.com/articles/nprot.2014.039.pdf

Reference72 articles.

1. Rasmussen, S.G. et al. Structure of a nanobody-stabilized active state of the β2 adrenoceptor. Nature 469, 175–180 (2011).

2. Rasmussen, S.G. et al. Crystal structure of the β2 adrenergic receptor-Gs protein complex. Nature 477, 549–555 (2011).

3. Low, C. et al. Nanobody mediated crystallization of an archeal mechanosensitive channel. PLoS ONE 8, e77984 (2013).

4. Baranova, E. et al. SbsB structure and lattice reconstruction unveil Ca2+ triggered S-layer assembly. Nature 487, 119–122 (2012).

5. Park, Y.J., Pardon, E., Wu, M., Steyaert, J. & Hol, W.G. Crystal structure of a heterodimer of editosome interaction proteins in complex with two copies of a cross-reacting nanobody. Nucleic Acids Res. 40, 1828–1840 (2012).

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