A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies-Reference-Cited by-同舟云学术

A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies

Published:2016-07-28 Issue:8 Volume:11 Page:1554-1571
ISSN:1754-2189
Container-title:Nature Protocols
language:en
Short-container-title:Nat Protoc

Author:

Magnani Francesca,Serrano-Vega Maria J,Shibata Yoko,Abdul-Hussein Saba,Lebon Guillaume,Miller-Gallacher Jennifer^ORCID,Singhal Ankita,Strege Annette,Thomas Jennifer A^ORCID,Tate Christopher G^ORCID

Publisher

Springer Science and Business Media LLC

Subject

General Biochemistry, Genetics and Molecular Biology

Link

http://www.nature.com/articles/nprot.2016.088.pdf

Reference82 articles.

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3. Drew, D. et al. A scalable, GFP-based pipeline for membrane protein overexpression screening and purification. Protein Sci. 14, 2011–2017 (2005).

4. Hattori, M., Hibbs, R.E. & Gouaux, E. A fluorescence-detection size-exclusion chromatography-based thermostability assay for membrane protein precrystallization screening. Structure 20, 1293–1299 (2012).

5. Kawate, T. & Gouaux, E. Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins. Structure 14, 673–681 (2006).

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