Macrophage-mediated trogocytosis contributes to destroying human schistosomes in a non-susceptible rodent host, Microtus fortis
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Published:2023-10-05
Issue:1
Volume:9
Page:
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ISSN:2056-5968
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Container-title:Cell Discovery
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language:en
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Short-container-title:Cell Discov
Author:
Shen Jia, Zhao Siyu, Peng Mei, Li Yanguo, Zhang Lichao, Li Xiaoping, Hu Yunyi, Wu Mingrou, Xiang Suoyu, Wu Xiaoying, Liu Jiahua, Zhang Beibei, Chen Zebin, Lin Datao, Liu Huanyao, Tang Wenyan, Chen JunORCID, Sun Xi, Liao Qi, Hide GeoffORCID, Zhou Zhijun, Lun Zhao-Rong, Wu Zhongdao
Abstract
AbstractSchistosoma parasites, causing schistosomiasis, exhibit typical host specificity in host preference. Many mammals, including humans, are susceptible to infection, while the widely distributed rodent, Microtus fortis, exhibits natural anti-schistosome characteristics. The mechanisms of host susceptibility remain poorly understood. Comparison of schistosome infection in M. fortis with the infection in laboratory mice (highly sensitive to infection) offers a good model system to investigate these mechanisms and to gain an insight into host specificity. In this study, we showed that large numbers of leukocytes attach to the surface of human schistosomes in M. fortis but not in mice. Single-cell RNA-sequencing analyses revealed that macrophages might be involved in the cell adhesion, and we further demonstrated that M. fortis macrophages could be mediated to attach and kill schistosomula with dependence on Complement component 3 (C3) and Complement receptor 3 (CR3). Importantly, we provided direct evidence that M. fortis macrophages could destroy schistosomula by trogocytosis, a previously undescribed mode for killing helminths. This process was regulated by Ca2+/NFAT signaling. These findings not only elucidate a novel anti-schistosome mechanism in M. fortis but also provide a better understanding of host parasite interactions, host specificity and the potential generation of novel strategies for schistosomiasis control.
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Genetics,Molecular Biology,Biochemistry
Reference76 articles.
1. Wells, K. & Clark, N. J. Host specificity in variable environments. Trends Parasitol. 35, 452–465 (2019). 2. Poulin, R., Krasnov, B. R. & Morand, S. Patterns of host specificity in parasites exploiting small mammals. Micromammals and Macroparasites: From Evolutionary Ecology To Management, 233–256 (Springer, 2006). 3. WHO. Schistosomiasis. www.who.int/news-room/fact-sheets/detail/schistosomiasis (WHO, 2023). 4. Cioli, D., Knopf, P. M. & Senft, A. W. A study of Schistosoma mansoni transferred into permissive and nonpermissive hosts. Int. J. Parasitol. 7, 293–297 (1977). 5. He, Y. et al. Immunological characteristics of natural resistance in Microtus fortis to infection with Schistosoma japonicum. Chin. Med. J. 112, 649–654 (1999).
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