descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques-Reference-Cited by-同舟云学术

descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques

Published:2024-06-12 Issue:1 Volume:15 Page:
ISSN:2041-1723
Container-title:Nature Communications
language:en
Short-container-title:Nat Commun

Author:

Otomo Kohei^ORCID,Omura Takaki,Nozawa Yuki^ORCID,Edwards Steven J.,Sato Yukihiko,Saito Yuri,Yagishita Shigehiro,Uchida Hitoshi^ORCID,Watakabe Yuki^ORCID,Naitou Kiyotada^ORCID,Yanai Rin,Sahara Naruhiko,Takagi Satoshi^ORCID,Katayama Ryohei^ORCID,Iwata Yusuke^ORCID,Shiokawa Toshiro,Hayakawa Yoku,Otsuka Kensuke,Watanabe-Takano Haruko^ORCID,Haneda Yuka^ORCID,Fukuhara Shigetomo^ORCID,Fujiwara Miku,Nii Takenobu,Meno Chikara^ORCID,Takeshita Naoki^ORCID,Yashiro Kenta,Rosales Rocabado Juan Marcelo^ORCID,Kaku Masaru^ORCID,Yamada Tatsuya^ORCID,Oishi Yumiko,Koike Hiroyuki^ORCID,Cheng Yinglan,Sekine Keisuke^ORCID,Koga Jun-ichiro^ORCID,Sugiyama Kaori,Kimura Kenichi^ORCID,Karube Fuyuki,Kim Hyeree^ORCID,Manabe Ichiro^ORCID,Nemoto Tomomi^ORCID,Tainaka Kazuki^ORCID,Hamada Akinobu,Brismar Hjalmar^ORCID,Susaki Etsuo A.^ORCID

Abstract

AbstractDespite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced (https://github.com/dbsb-juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies.

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41467-024-49131-1.pdf

Reference70 articles.

1. Dodt, H. U. et al. Ultramicroscopy: three-dimensional visualization of neuronal networks in the whole mouse brain. Nat. Methods 4, 331–336 (2007).

2. Dent, J. A., Polson, A. G. & Klymkowsky, M. W. A whole-mount immunocytochemical analysis of the expression of the intermediate filament protein vimentin in Xenopus. Development 105, 61–74 (1989).

3. Klingberg, A. et al. Fully automated evaluation of total glomerular number and capillary tuft size in nephritic kidneys using lightsheet microscopy. J. Am. Soc. Nephrol. 28, 452–459 (2017).

4. Ertürk, A. et al. Three-dimensional imaging of solvent-cleared organs using 3DISCO. Nat. Protoc. 7, 1983–1995 (2012).

5. Susaki, E. A. et al. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis. Cell 157, 726–739 (2014).