Archaeal DNA-import apparatus is homologous to bacterial conjugation machinery

Author:

Beltran Leticia C.,Cvirkaite-Krupovic Virginija,Miller Jessalyn,Wang Fengbin,Kreutzberger Mark A. B.,Patkowski Jonasz B.ORCID,Costa Tiago R. D.ORCID,Schouten Stefan,Levental IlyaORCID,Conticello Vincent P.ORCID,Egelman Edward H.ORCID,Krupovic MartORCID

Abstract

AbstractConjugation is a major mechanism of horizontal gene transfer promoting the spread of antibiotic resistance among human pathogens. It involves establishing a junction between a donor and a recipient cell via an extracellular appendage known as the mating pilus. In bacteria, the conjugation machinery is encoded by plasmids or transposons and typically mediates the transfer of cognate mobile genetic elements. Much less is known about conjugation in archaea. Here, we determine atomic structures by cryo-electron microscopy of three conjugative pili, two from hyperthermophilic archaea (Aeropyrum pernixandPyrobaculum calidifontis) and one encoded by the Ti plasmid of the bacteriumAgrobacterium tumefaciens, and show that the archaeal pili are homologous to bacterial mating pili. However, the archaeal conjugation machinery, known as Ced, has been ‘domesticated’, that is, the genes for the conjugation machinery are encoded on the chromosome rather than on mobile genetic elements, and mediates the transfer of cellular DNA.

Funder

U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences

Publisher

Springer Science and Business Media LLC

Subject

General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary

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