Aegilops sharonensis genome-assisted identification of stem rust resistance gene Sr62
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Published:2022-03-25
Issue:1
Volume:13
Page:
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ISSN:2041-1723
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Container-title:Nature Communications
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language:en
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Short-container-title:Nat Commun
Author:
Yu Guotai, Matny OadiORCID, Champouret Nicolas, Steuernagel BurkhardORCID, Moscou Matthew J.ORCID, Hernández-Pinzón InmaculadaORCID, Green PhonORCID, Hayta SadiyeORCID, Smedley MarkORCID, Harwood WendyORCID, Kangara Ngonidzashe, Yue Yajuan, Gardener Catherine, Banfield Mark J.ORCID, Olivera Pablo D., Welchin Cole, Simmons Jamie, Millet Eitan, Minz-Dub Anna, Ronen Moshe, Avni RazORCID, Sharon AmirORCID, Patpour MehranORCID, Justesen Annemarie F., Jayakodi MurukarthickORCID, Himmelbach Axel, Stein NilsORCID, Wu Shuangye, Poland JesseORCID, Ens JenniferORCID, Pozniak CurtisORCID, Karafiátová MiroslavaORCID, Molnár István, Doležel JaroslavORCID, Ward Eric R.ORCID, Reuber T. Lynne, Jones Jonathan D. G.ORCID, Mascher MartinORCID, Steffenson Brian J.ORCID, Wulff Brande B. H.ORCID
Abstract
AbstractThe wild relatives and progenitors of wheat have been widely used as sources of disease resistance (R) genes. Molecular identification and characterization of these R genes facilitates their manipulation and tracking in breeding programmes. Here, we develop a reference-quality genome assembly of the wild diploid wheat relative Aegilops sharonensis and use positional mapping, mutagenesis, RNA-Seq and transgenesis to identify the stem rust resistance gene Sr62, which has also been transferred to common wheat. This gene encodes a tandem kinase, homologues of which exist across multiple taxa in the plant kingdom. Stable Sr62 transgenic wheat lines show high levels of resistance against diverse isolates of the stem rust pathogen, highlighting the utility of Sr62 for deployment as part of a polygenic stack to maximize the durability of stem rust resistance.
Funder
RCUK | Biotechnology and Biological Sciences Research Council
Publisher
Springer Science and Business Media LLC
Subject
General Physics and Astronomy,General Biochemistry, Genetics and Molecular Biology,General Chemistry,Multidisciplinary
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