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2. Sucrose, 10.0 gm; L-asparagine, 1.0 gm; KH2PO4, 0.5 gm; MgSO4.7H2O, 0.25 gm; trace element solution, 1 ml. ; thiamine hydrochloride, 1 mg; water, 1 litre. The trace element solution contains Na2B4O7.10H2O, 88 mg; CuSO4.5H2O, 393 mg; Fe2(SO4)3.5H2O, 910 mg; MnCl2.4H2O, 72 mg; Na2MoO4.2H20, 50 mg; ZnSO4.7H2O, 4,403 mg; EDTA, 5gm; water, 1 l. Difco bacto agar or Oxoid agar No. 3, 0.8 per cent.
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