Author:
Kosheleva Nastasia V.,Efremov Yuri M.,Shavkuta Boris S.,Zurina Irina M.,Zhang Deying,Zhang Yuanyuan,Minaev Nikita V.,Gorkun Anastasiya A.,Wei Shicheng,Shpichka Anastasia I.,Saburina Irina N.,Timashev Peter S.
Abstract
AbstractBiological self-assembly is crucial in the processes of development, tissue regeneration, and maturation of bioprinted tissue-engineered constructions. The cell aggregates—spheroids—have become widely used model objects in the study of this phenomenon. Existing approaches describe the fusion of cell aggregates by analogy with the coalescence of liquid droplets and ignore the complex structural properties of spheroids. Here, we analyzed the fusion process in connection with structure and mechanical properties of the spheroids from human somatic cells of different phenotypes: mesenchymal stem cells from the limbal eye stroma and epithelial cells from retinal pigment epithelium. A nanoindentation protocol was applied for the mechanical measurements. We found a discrepancy with the liquid drop fusion model: the fusion was faster for spheroids from epithelial cells with lower apparent surface tension than for mesenchymal spheroids with higher surface tension. This discrepancy might be caused by biophysical processes such as extracellular matrix remodeling in the case of mesenchymal spheroids and different modes of cell migration. The obtained results will contribute to the development of more realistic models for spheroid fusion that would further provide a helpful tool for constructing cell aggregates with required properties both for fundamental studies and tissue reparation.
Funder
Russian Science Foundation
the Research project "The study of mechanisms of aging and regeneration and development of conditions for obtaining tissue-engineered constructs using 2D and 3D cell cultures"
the Russian academic excellence project ‘5-100’
Russian Foundation for Basic Research
Publisher
Springer Science and Business Media LLC
Cited by
45 articles.
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