Author:
Ishiki Kengo,Yamashita Kazuto,Watanabe Shunsuke,Miura Masahiro,Kawahira Junko,Arimatsu Yuji,Kawasaki Kana,Iwanaga Shigeki,Sato Toshiyuki
Abstract
AbstractPlasma β-amyloid (Aβ) assays are a promising tool for Alzheimer’s disease diagnosis in clinical practice. To obtain reliable results, establishing an appropriate sample-handling procedure for each analytical platform is warranted. This study proposes an appropriate sample-handling procedure using HISCL analyzer by elucidating the individual/combined effects of pre-analytical parameters on plasma Aβ42/Aβ40 levels. We investigated the effects of various pre-analytical parameters, including storage times for whole blood, plasma, and freezing conditions, on plasma Aβ42/Aβ40 levels, and confirmed if these values met the acceptable criteria. Plasma Aβ42/Aβ40 levels were acceptable in all conditions. We determined our protocol by confirming that plasma Aβ42/Aβ40 levels remained acceptable when combining pre-analytical parameters. We established an appropriate sample-handling protocol that ensures reliable measurement of plasma Aβ42/Aβ40 levels using HISCL analyzer. We believe the Aβ assay, with our protocol, shows promise for aiding AD diagnosis in clinical settings.
Publisher
Springer Science and Business Media LLC
Reference36 articles.
1. Gauthier, S., Webster, C., Servaes, S., Morais, J. A., & Rosa-Neto, P. World Alzheimer Report 2022: Life after diagnosis: Navigating treatment, care and support. London, England: Alzheimer’s Disease International. https://www.alzint.org/u/World-Alzheimer-Report-2022.pdf. (2022).
2. World Health Organization. Global action plan on the public health response to dementia 2017–2025. https://www.who.int/publications/i/item/global-action-plan-on-the-public-health-response-to-dementia-2017---2025. (2017).
3. Vaz, M. & Silvestre, S. Alzheimer’s disease: recent treatment strategies. Eur. J. Pharmacol. 887, 173554 (2020).
4. Iwatsubo, T. et al. Visualization of A beta 42(43) and A beta 40 in senile plaques with end-specific A beta monoclonals: evidence that an initially deposited species is A beta 42(43). Neuron 13, 45–53 (1994).
5. Rowe, C. C. et al. Amyloid imaging results from the Australian Imaging, Biomarkers and Lifestyle (AIBL) study of aging. Neurobiol. Aging 31, 1275–1283 (2010).