Author:
Rawal Kamal,Sinha Robin,Abbasi Bilal Ahmed,Chaudhary Amit,Nath Swarsat Kaushik,Kumari Priya,Preeti P.,Saraf Devansh,Singh Shachee,Mishra Kartik,Gupta Pranjay,Mishra Astha,Sharma Trapti,Gupta Srijanee,Singh Prashant,Sood Shriya,Subramani Preeti,Dubey Aman Kumar,Strych Ulrich,Hotez Peter J.,Bottazzi Maria Elena
Abstract
AbstractAntigen identification is an important step in the vaccine development process. Computational approaches including deep learning systems can play an important role in the identification of vaccine targets using genomic and proteomic information. Here, we present a new computational system to discover and analyse novel vaccine targets leading to the design of a multi-epitope subunit vaccine candidate. The system incorporates reverse vaccinology and immuno-informatics tools to screen genomic and proteomic datasets of several pathogens such as Trypanosoma cruzi, Plasmodium falciparum, and Vibrio cholerae to identify potential vaccine candidates (PVC). Further, as a case study, we performed a detailed analysis of the genomic and proteomic dataset of T. cruzi (CL Brenner and Y strain) to shortlist eight proteins as possible vaccine antigen candidates using properties such as secretory/surface-exposed nature, low transmembrane helix (< 2), essentiality, virulence, antigenic, and non-homology with host/gut flora proteins. Subsequently, highly antigenic and immunogenic MHC class I, MHC class II and B cell epitopes were extracted from top-ranking vaccine targets. The designed vaccine construct containing 24 epitopes, 3 adjuvants, and 4 linkers was analysed for its physicochemical properties using different tools, including docking analysis. Immunological simulation studies suggested significant levels of T-helper, T-cytotoxic cells, and IgG1 will be elicited upon administration of such a putative multi-epitope vaccine construct. The vaccine construct is predicted to be soluble, stable, non-allergenic, non-toxic, and to offer cross-protection against related Trypanosoma species and strains. Further, studies are required to validate safety and immunogenicity of the vaccine.
Funder
Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation
Science and Engineering Research Board
Publisher
Springer Science and Business Media LLC
Cited by
38 articles.
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