Diospyros montana mediated reduction, stabilization, and characterization of silver nanoparticles and evaluation of their mosquitocidal potentiality against dengue vector Aedes albopictus

Abstract

AbstractRecent research has focused on nanoparticles. Aedes albopictus is a potential vector that transmits fatal diseases. Recently, Phyto-reduced silver nanoparticles (AgNPs) were shown to be mosquito larvicides. This study aimed to synthesize silver nanoparticles using Diospyros montana leaf extract, characterize them, and test their efficacy as larvicide and pupicide against Ae. albopictus mosquitoes, determine their duration of effectiveness as a larvicide, identify plant compounds that help to synthesize nanoparticles, and assess their effects on non-target organisms. Quercetin, luteolin, kaempferol, gallocatechin gallate, epigallocatechin gallate, and capsaicin are among the novel reducing and capping agents found in D. montana leaf through LCMS analysis. The color shift and distinctive peak in UV–Vis spectroscopy made it simple to see how biogenic AgNPs were produced by converting Ag+ ions into Ag0. Substantial negative value (− 19.10 mv) of zeta potential demonstrated the long-term stability of AgNPs. A moderate range (8.72 − 50.75 nm) of particle size distribution pattern was obtained using the DLS technique. SEM and TEM images depicted the quasi-spherical (or polyhedral) and spherical shape of the nanoparticles, having approximately 16.75 nm average size. Synthesized AgNPs had a low LC90 value (< 10 ppm) for all larval instars and pupae of Ae. albopictus and had negligible mal effect on non-target organisms. Regression equations showed dose-dependent mortality by the positive correlation between mortality rate and AgNPs concentration, and each time the regression coefficient (R2) value was larger than zero. This study shows that D. montana leaf extract is an environment-friendly and sustainable source of an effective reducing and capping agent to synthesize highly stable, ecologically acceptable silver nanoparticles and their application as mosquitocide.