Abstract
AbstractLongitudinal in vivo micro-computed tomography (micro-CT) is of interest to non-invasively capture the healing process of individual animals in preclinical fracture healing studies. However, it is not known whether longitudinal imaging itself has an impact on callus formation and remodeling. In this study, a scan group received weekly micro-CT measurements (week 0–6), whereas controls were only scanned post-operatively and at week 5 and 6. Registration of consecutive scans using a branching scheme (bridged vs. unbridged defect) combined with a two-threshold approach enabled assessment of localized bone turnover and mineralization kinetics relevant for monitoring callus remodeling. Weekly micro-CT application did not significantly change any of the assessed callus parameters in the defect and periosteal volumes. This was supported by histomorphometry showing only small amounts of cartilage residuals in both groups, indicating progression towards the end of the healing period. Also, immunohistochemical staining of Sclerostin, previously associated with mediating adverse radiation effects on bone, did not reveal differences between groups. The established longitudinal in vivo micro-CT-based approach allows monitoring of healing phases in mouse femur defect models without significant effects of anesthesia, handling and radiation on callus properties. Therefore, this study supports application of longitudinal in vivo micro-CT for healing-phase-specific monitoring of fracture repair in mice.
Publisher
Springer Science and Business Media LLC
Reference67 articles.
1. Taiani, J. T. et al. Embryonic stem cell therapy improves bone quality in a model of impaired fracture healing in the mouse; tracked temporally using in vivo micro-CT. Bone 64, 263–272 (2014).
2. Locher, R. J. et al. Traumatic brain injury and bone healing: radiographic and biomechanical analyses of bone formation and stability in a combined murine trauma model. J. Musculoskel. Neuron. 15, 309–315 (2015).
3. Graef, F. et al. Impaired fracture healing with high non-union rates remains irreversible after traumatic brain injury in leptin-deficient mice. J. Musculoskel. Neuron. 17, 78–85 (2017).
4. Mehta, M., Checa, S., Lienau, J., Hutmacher, D. & Duda, G. N. In vivo tracking of segmental bone defect healing reveals that callus patterning is related to early mechanical stimuli. Eur. Cell Mater. 24, 358–371 (2012).
5. Tourolle né Betts, D. C. et al. The association between mineralised tissue formation and the mechanical local in vivo environment: Time-lapsed quantification of a mouse defect healing model. Preprint at https://doi.org/10.1101/721365 (2019).
Cited by
44 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献