Author:
Farooq Naila,Ather Laraib,Shafiq Muhammad,Nawaz-ul-Rehman Muhammad Shah,Haseeb Muhammad,Anjum Tehmina,Abbas Qamar,Hussain Mujahid,Ali Numan,Asad Abbas Syed Agha Armaghan,Mushtaq Sehrish,Haider Muhammad Saleem,Sadiq Saleha,Shahid Muhammad Adnan
Abstract
AbstractClimate change, pesticide resistance, and the need for developing new plant varieties have galvanized biotechnologists to find new solutions in order to produce transgenic plants. Over the last decade scientists are working on green metallic nanoparticles to develop DNA delivery systems for plants. In the current study, green Iron nanoparticles were synthesized using leaf extract of Camellia sinensis (green tea) and Iron Chloride (FeCl3), the characterization and Confirmation was done using UV–VIS Spectroscopy, FTIR, SEM, and TEM. Using these nanoparticles, a novel method of gene transformation in okra plants was developed, with a combination of different Magnetofection factors. Maximum gene transformation efficiency was observed at the DNA to Iron-nanoparticles ratio of 1:20, by rotation of mixture (Plasmid DNA, Iron-nanoparticles, and seed embryo) at 800 rpm for 5 h. Using this approach, the transformation of the GFP (green fluorescent protein) gene was successfully carried out in Abelmoschus esculentus (Okra plant). The DNA transformation was confirmed by observing the expression of transgene GFP via Laser Scanning Confocal Microscope (LSCM) and PCR. This method is highly economical, adaptable, genotype independent, eco-friendly, and time-saving as well. We infer that this approach can be a potential solution to combat the yield and immunity challenges of plants against pathogens.
Publisher
Springer Science and Business Media LLC
Reference53 articles.
1. Klein, T. M. & Jones, T. J. Methods of Genetic Transformation: The Gene Gun in Molecular Improvement of Cereal Crops 21–42 (Springer, 1999).
2. Fromm, M. E., Taylor, L. P. & Walbot, V. Stable transformation of maize after gene transfer by electroporation. Nature 319(6056), 791–793 (1986).
3. Rivera, A. L. et al. Physical methods for genetic plant transformation. Phys. Life Rev. 9(3), 308–345 (2012).
4. Masani, M. Y. A. et al. Efficient transformation of oil palm protoplasts by PEG-mediated transfection and DNA microinjection. PLoS ONE 9(5), e96831 (2014).
5. Zhao, X. et al. Pollen magnetofection for genetic modification with magnetic nanoparticles as gene carriers. Nat. Plants 3(12), 956–964 (2017).
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