Author:
Sugita Sachi,Yamato Masanori,Hatabu Toshimitsu,Kataoka Yosky
Abstract
AbstractA high rate of glycolysis, one of the most common features of cancer, is used in positron emission tomography (PET) imaging to visualize tumor tissues using 18F-fluorodeoxyglucose (18F-FDG). Heterogeneous intratumoral distribution of 18F-FDG in tissues has been established in some types of cancer, and the maximum standardized uptake value (SUVmax) has been correlated with poor prognosis. However, the phenotype of cells that show high 18F-FDG accumulation in tumors remains unknown. Here, we combined quantitative micro-autoradiography with fluorescence immunohistochemistry to simultaneously visualize 18F-FDG distribution, the expression of multiple proteins, and hypoxic regions in the cancer microenvironment of a human A431 xenograft tumor in C.B-17/Icr-scid/scid mice. We found that the highest 18F-FDG accumulation was in cancer-derived cells undergoing epithelial-mesenchymal transition (EMT) in hypoxic regions, implicating these regions as a major contributor to increased glucose metabolism, as measured by 18F-FDG-PET.
Publisher
Springer Science and Business Media LLC
Reference51 articles.
1. Mueckler, M. & Thorens, B. The SLC2 (GLUT) family of membrane transporters. Mol. Aspects Med. 34, 121–138 (2013).
2. Maschauer, S., Prante, O., Hoffmann, M., Deichen, J. T. & Kuwert, T. Characterization of 18F-FDG uptake in human endothelial cells in vitro. J. Nucl. Med. 45, 455–460 (2004).
3. Zasadny, K. R. & Wahl, R. L. Standardized uptake values of normal tissues at PET with 2-[fluorine-18]-fluoro-2-deoxy-D-glucose: variations with body weight and a method for correction. Radiology 189, 847–850 (1993).
4. Lodge, M. A. Repeatability of SUV in Oncologic (18)F-FDG PET. J. Nucl. Med. 58, 523–532 (2017).
5. de Langen, A. J. et al. Repeatability of 18F-FDG uptake measurements in tumors: a metaanalysis. J. Nucl. Med. 53, 701–708 (2012).
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献