Abstract
AbstractTo explore the hub comorbidity genes and potential pathogenic mechanisms of hypopharyngeal carcinoma with esophageal carcinoma, and evaluate their diagnostic value for hypopharyngeal carcinoma with co-morbid esophageal carcinoma. We performed gene sequencing on tumor tissues from 6 patients with hypopharyngeal squamous cell carcinoma with esophageal squamous cell carcinoma (hereafter referred to as “group A”) and 6 patients with pure hypopharyngeal squamous cell carcinoma (hereafter referred to as “group B”). We analyzed the mechanism of hub genes in the development and progression of hypopharyngeal squamous cell carcinoma with esophageal squamous cell carcinoma through bioinformatics, and constructed an ROC curve and Nomogram prediction model to analyze the value of hub genes in clinical diagnosis and treatment. 44,876 genes were sequenced in 6 patients with group A and 6 patients with group B. Among them, 76 genes showed significant statistical differences between the group A and the group B.47 genes were expressed lower in the group A than in the group B, and 29 genes were expressed higher. The top five hub genes were GABRG2, CACNA1A, CNTNAP2, NOS1, and SCN4B. GABRG2, CNTNAP2, and SCN4B in the hub genes have high diagnostic value in determining whether hypopharyngeal carcinoma patients have combined esophageal carcinoma (AUC: 0.944, 0.944, 0.972). These genes could possibly be used as potential molecular markers for assessing the risk of co-morbidity of hypopharyngeal carcinoma combined with esophageal carcinoma.
Publisher
Springer Science and Business Media LLC
Reference38 articles.
1. Kawakita, D. et al. Trends in the incidence of head and neck cancer by subsite between 1993 and 2015 in Japan. Cancer Med. 11(6), 1553–1560 (2022).
2. Visini, M., Giger, R., Shelan, M., Elicin, O. & Anschuetz, L. Predicting factors for oncological and functional outcome in hypopharyngeal carcinoma. Laryngoscope 131(5), E1543–E1549 (2021).
3. Lee, D. H. et al. Second cancer incidence, risk factor, and specific mortality in head and neck squamous cell carcinoma. Otolaryngol. Head Neck Surg. 149(4), 579–586 (2013).
4. Committee of esophageal carcinoma in China Anti-Cancer A, Chinese Working Group on Cooperative D, Treatment of H, Esophageal C. Chinese expert consensus on multiple primary cancers of hypopharynx and esophagus. Zhonghua Wai Ke Za Zhi. 58(8), 589–595 (2020).
5. Thompson, C. S. G. et al. Complications and predisposing factors from a decade of total laryngectomy. J. Laryngol. Otol. 134(3), 256–262 (2020).