Author:
Yuan Si,Deng Bing,Ye Qiuping,Wu Zhennan,Wu Junshang,Wang Lin,Xu Qin,Yao Lulu,Xu Nenggui
Abstract
AbstractParaventricular hypothalamus (PVH) is demonstrated to regulate stress, feeding behaviors, and other related homeostatic processes. However, no direct evidence has been investigated for the role of PVH in swallowing function. Acupuncture therapy at Lianquan (CV23) acupoint has been reported to improve the swallowing function in clinical trials, but its underlying mechanism still needs to be uncovered. Thus, we aimed to explore whether PVH involved the acupuncture mediated regulating swallowing function. Chemogenetics, electromyography (EMG) recording, and immunofluorescence staining methods were combined to demonstrate that neurons in PVH could be activated by electroacupuncture (EA) stimulation at CV23, and this neuronal cluster was represented as excitatory neurons. Furthermore, we mapped both the inputs and outputs of PVH neurons using viral tracing. The neurons in PVH projected with the brain regions, including parabrachial nucleus (PBN) and the solitary tract nucleus (NTS), which both participated in the swallowing process. The EA function regulating the swallowing was attenuated after inhibiting the neurons in PVH in the post stroke dysphagia. In conclusion, this study suggested that EA at CV23 could regulate swallowing function involving the excitatory neurons in PVH.
Funder
the Youth Program of the National Natural Science Foundation of China
the General Program of the National Natural Science Foundation of China
the Opening Operation Program of Key Laboratory of Acupuncture and Moxibustion of Traditional Chinese Medicine in Guangdong
the Key laboratory Program of universities in Guangdong province
the National Key R&D Program of China
the Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program
Publisher
Springer Science and Business Media LLC
Cited by
5 articles.
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