Author:
Otagiri Takuma,Shiga Yasuhiro,Hozumi Takashi,Matsuura Yusuke,Tajiri Ikuko,Takayama Naoya,Alejandra Maria,Shiko Yuki,Orita Sumihisa,Inage Kazuhide,Eguchi Yawara,Suzuki Takane,Suzuki-Narita Miyako,Mukai Michiaki,Mukaihata Tomohito,Tsuchiya Ryuto,Tokeshi Soichiro,Okuyama Kohei,Arai Takahito,Toshi Noriyasu,Furuya Takeo,Maki Satoshi,Aoki Yasuchika,Ohtori Seiji
Abstract
AbstractPlatelet-rich plasma (PRP) promotes bone union through osteoinduction. We investigated whether adding demineralized bone matrix (DBM), derived naturally from biomaterial and with various growth factors, for osteoconductivity and bone marrow fluid for osteogenesis results in different bone unions. Eight-week-old male Sprague–Dawley rats were divided into four groups of five based on transplantation material: sham control (C group); DBM alone (D group); DBM + PRP (DP group); and DBM + PRP + bone marrow fluid (DPB group). After posterolateral fusion at L3-5, postoperative weekly CT imaging determined average number of bone union in facet joints (4 joints × 5 animals = 20 joints) and bone formation. Pathological evaluation and bone strength were assessed using 3-point bending two weeks postoperatively. Facet joint bone union at four weeks postoperatively was 4/20 (20%, DP group) and 8/20 (40%, DPB group) joints. Six weeks postoperatively, it was 7/20 (35%, D group), 12/20 (60%, DP group), and 16/20 (80%, DPB group). Eight weeks postoperatively, it was 13/20 (65%, D group), 17/20 (85%, DP group), and 20/20 (100%, DPB group), suggesting that DPB > DP > D > C. Bone formation and bone strength showed a similar DPB > DP > D > C group trend. Adding PRP and bone marrow fluid to DBM promotes bone union and strength.
Publisher
Springer Science and Business Media LLC