Author:
Konecka Edyta,Szymkowiak Paweł
Abstract
AbstractWolbachia (phylum Pseudomonadota, class Alfaproteobacteria, order Rickettsiales, family Ehrlichiaceae) is a maternally inherited bacterial symbiont infecting more than half of arthropod species worldwide and constituting an important force in the evolution, biology, and ecology of invertebrate hosts. Our study contributes to the limited knowledge regarding the presence of intracellular symbiotic bacteria in spiders. Specifically, we investigated the occurrence of Wolbachia infection in the spider species Enoplognatha latimana Hippa and Oksala, 1982 (Araneae: Theridiidae) using a sample collected in north-western Poland. To the best of our knowledge, this is the first report of Wolbachia infection in E. latimana. A phylogeny based on the sequence analysis of multiple genes, including 16S rRNA, coxA, fbpA, ftsZ, gatB, gltA, groEL, hcpA, and wsp revealed that Wolbachia from the spider represented supergroup A and was related to bacterial endosymbionts discovered in other spider hosts, as well as insects of the orders Diptera and Hymenoptera. A sequence unique for Wolbachia supergroup A was detected for the ftsZ gene. The sequences of Wolbachia housekeeping genes have been deposited in publicly available databases and are an important source of molecular data for comparative studies. The etiology of Wolbachia infection in E. latimana is discussed.
Publisher
Springer Science and Business Media LLC
Reference106 articles.
1. Ahmed, M. Z., Ajauro-Jnr, E. V., Welchm, J. J. & Kawahara, A. Y. Wolbachia in butterflies and moths: Geographic structure in infection frequency. Front. Zool. 12(16), 1–9 (2015).
2. Weinert, L. A., Araujo-Jnr, E. V., Ahmed, M. Z. & Welch, J. J. The incidence of bacterial endosymbionts in terrestrial arthropods. Proc. R. Soc. B Biol. Sci. 282, 20150249 (2015).
3. Sazama, E. J., Bosch, M. J., Shouldis, C. S., Ouellette, S. P. & Wesner, J. S. Incidence of Wolbachia in aquatic insects. Ecol. Evol. 7, 1165–1169 (2017).
4. Kajtoch, Ł et al. Using host species traits to understand the Wolbachia infection distribution across terrestrial beetles. Sci. Rep. 9, 847 (2019).
5. Sazama, E. J., Ouellette, S. P. & Wesner, J. S. Bacterial endosymbionts are common among, but not necessarily within, insect species. Environ. Entomol. 48(1), 127–133 (2019).