Author:
Gu Jian,Duane Brett,Repin Mikhail,Brenner David J.,Zenhausern Frederic
Abstract
AbstractWe report a shipping container that enables a disruptive logistics for cytogenetic biodosimetry for radiation countermeasures through pre-processing cell culture during transportation. The container showed precise temperature control (< 0.01 °C) with uniform sample temperature (< 0.1 °C) to meet the biodosimetry assay requirements. Using an existing insulated shipping box and long shelf life alkaline batteries makes it ideal for national stockpile. Dose curve of cytogenetic biodosimetry assay using the shipping container showed clear dose response and high linear correlation with the control dose curve using a laboratory incubator (Pearson’s correlation coefficient: 0.992). The container’s ability of pre-processing biological samples during transportation could have a significant impact on radiation countermeasure, as well as potential impacts in other applications such as biobanking, novel molecular or cell-based assays or therapies.
Funder
National Institute of Allergy and Infectious Diseases, United States
Publisher
Springer Science and Business Media LLC
Reference22 articles.
1. Grace, M. B. et al. Rapid radiation dose assessment for radiological public health emergencies: roles of NIAID and BARDA. Health Phys. 98(2), 172–178. https://doi.org/10.1097/01.HP.0000348001.60905.c0 (2010).
2. https://en.wikipedia.org/wiki/Nuclear_and_radiation_accidents_and_incidents [Cited 2020 10/19].
3. Sullivan, J. M. et al. Assessment of biodosimetry methods for a mass-casualty radiological incident: medical response and management considerations. Health Phys. 105(6), 540–554. https://doi.org/10.1097/HP.0b013e31829cf221 (2013).
4. Cytogenetic Dosimetry: Applications in Preparedness for and Response to Radiation Emergencies, IAEA https://www.iaea.org/publications/8735/cytogenetic-dosimetry-applications-in-preparedness-for-and-response-to-radiationemergencies (2011).
5. Repin, M., Pampou, S., Karan, C., Brenner, D. J. & Garty, G. RABiT-II: implementation of a high-throughput micronucleus biodosimetry assay on commercial biotech robotic systems. Radiat. Res. 187(4), 492–498. https://doi.org/10.1667/rr011cc.1 (2017).
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献