Author:
Zhang Tianlong,Li Haoran,Sun Shiwei,Zhou Wuling,Zhang Tieqi,Yu Yueming,Wang Qiang,Wang Minghai
Abstract
AbstractFemoral head necrosis is responsible for severe pain and its incidence is increasing. Abnormal adipogenic differentiation and fat cell hypertrophy of bone marrow mesenchymal stem cells increase intramedullary cavity pressure, leading to osteonecrosis. By analyzing gene expression before and after adipogenic differentiation, we found that Microfibril-Associated Protein 5 (MFAP5) is significantly down-regulated in adipogenesis whilst the mechanism of MFAP5 in regulating the differentiation of bone marrow mesenchymal stem cells is unknown. The purpose of this study was to clarify the role of MAFP5 in adipogenesis and therefore provide a theoretical basis for future therapeutic options of osteonecrosis. By knockdown or overexpression of MFAP5 in C3H10 and 3T3-L1 cells, we found that MFAP5 was significantly down-regulated as a key regulator of adipogenic differentiation, and identified the underlying downstream molecular mechanism. MFAP5 directly bound to and inhibited the expression of Staphylococcal Nuclease And Tudor Domain Containing 1, an essential coactivator of PPARγ, exerting an important regulatory role in adipogenesis.
Funder
Natural Science Foundation of Shanghai
Natural Science Foundation of Minhang District Shanghai
the Key Department of Minhang District Shanghai
the Key Department of the Fifth People's Hospital of Shanghai
the Fifth People's Hospital of Shanghai, Fudan University
the Minhang District Leading Talent Development Funds
Publisher
Springer Science and Business Media LLC
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