Abstract
AbstractThe use of antibiotics with semen extender appears to be a practical solution to minimise bacterial growth in fresh boar semen preservation. Unfortunately, the excessive use of antibiotics promotes antimicrobial resistance (AMR). This becomes a worldwide concern due to the antimicrobial resistance genes transmitted to animals, environment, and humans. Probiotics are one of the alternative methods to reduce antibiotic use. They could inhibit pathogenic bacteria by producing antimicrobial substances in cell free supernatants (CFS). Nevertheless, there is no comprehensive study undertaken on inhibitory activity against pathogenic bacteria isolated from boar semen origin. Our study investigated the efficacy of CFS produced from selected probiotics: Bacillus spp., Enterococcus spp., Weissella spp., Lactobacillus spp., and Pediococcus spp. inhibiting pathogenic bacteria isolated from fresh boar semen. Besides, the semen-origin pathogenic bacteria are subjected to identification, antimicrobial resistance genes detection, and antibiotic susceptibility test (AST). Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis are the most common pathogens identified in boar semen with resistance to numerous antibiotics used in pig industry. The CFS with its antimicrobial peptides and/or bacteriocin constituent derived from selected probiotics could inhibit the growth of pathogenic bacteria carrying antimicrobial resistance genes (mcr-3 and int1 genes). The inhibition zones for Pseudomonas aeruginosa, Escherichia coli, and Proteus mirabilis provided more efficient results in the CFS derived from Lactobacillus spp. and Pediococcus spp. than those of the CFS produced from Enterococcus spp., Weissella spp. and Bacillus spp., respectively. It is worth noted that as the incubation time increased, the antibacterial activity decreased conversely. Our results on CFS with its antimicrobial peptides and/or bacteriocin constituent inhibits semen-origin pathogenic bacteria guide the direction as a promising alternative method used in the semen extender preservation of the pig industry.
Funder
National Research Council of Thailand
Publisher
Springer Science and Business Media LLC
Reference61 articles.
1. Maes, D. et al. Diseases in swine transmitted by artificial insemination: An overview. Theriogenology 70, 1337–1345. https://doi.org/10.1016/j.theriogenology.2008.06.018 (2008).
2. Kuster, C. E. & Althouse, G. C. The impact of bacteriospermia on boar sperm storage and reproductive performance. Theriogenology 85, 21–26. https://doi.org/10.1016/j.theriogenology.2015.09.049 (2016).
3. Farnum, D. W. & Riese, R. L. Urogenital infections in sows and gilts; differential diagnosis, diagnostic techniques and control. Iowa State Univ. Vet. 51, 1–5. https://core.ac.uk/reader/38906280 (1989).
4. de Winter, P., Verdoncka, M., de Kruif, A., Devriese, L. & Haesebrouck, F. Bacterial endometritis and vaginal discharge in the sow: Prevalence of different bacterial species and experimental reproduction of the syndrome. Anim. Reprod. Sci. 37, 325–335 (1995).
5. Lang, A., Kaeoket, K., Kindahl, H., Madej, A. & Einarsson, S. Influence of CRH and ACTH administration on endocrine profile and ovulation in sows. Reprod. Domest. Anim. 39, 181–189. https://doi.org/10.1111/j.1439-0531.2004.00501.x (2004).