Development of in-house ELISA based on recombinant gag proteins of small ruminant lentiviruses isolated from goats in Thailand-Reference-Cited by-同舟云学术

Development of in-house ELISA based on recombinant gag proteins of small ruminant lentiviruses isolated from goats in Thailand

Published:2024-02-13 Issue:1 Volume:14 Page:
ISSN:2045-2322
Container-title:Scientific Reports
language:en
Short-container-title:Sci Rep

Author:

Mongkonwattanaporn Tatchapon^ORCID,Lertwatcharasarakul Preeda^ORCID,Rukkwamsuk Theera^ORCID

Abstract

AbstractSmall ruminant lentiviruses (SRLVs), are grouped in Retroviridae family, remain a significant loss in the small ruminant husbandry. As a result of unavailability of vaccine and effective treatment, the diagnosis plays a crucial role for the control of SRLV infection. However, the major challenge of diagnosis of SRLV infection is the genetic and antigenic variability of the viruses that can lead to a failure in serological detection. This study investigated the circulating strains of the viruses in goats in Thailand and an in-house ELISA was developed. The coding sequences for gag protein were optimized, synthesized, and expressed in Escherichia coli for increasing the sensitivity of ELISA test. A total of 365 serum samples were examined against the recombinant protein in an in-house ELISA. The results showed that the recombinant gag achieves 96.67% sensitivity and 93.18% specificity as compared with the commercially available ELISA test kit.

Funder

National Research Council of Thailand

Publisher

Springer Science and Business Media LLC

Link

https://www.nature.com/articles/s41598-024-54360-x.pdf

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