3D scattering microphantom sample to assess quantitative accuracy in tomographic phase microscopy techniques

Author:

Krauze Wojciech,Kuś Arkadiusz,Ziemczonok Michał,Haimowitz Max,Chowdhury Shwetadwip,Kujawińska Małgorzata

Abstract

AbstractIn this paper we present a structurally-complex biomimetic scattering structure, fabricated with two-photon polymerization, and utilize this object in order to benchmark a computational imaging system. The phantom allows to tailor the scattering by modifying its degrees of freedom i.e. refractive index contrast and scattering layer dimensions and incorporates a 3D imaging quality test, representing a single cell within tissue. While the sample may be used with multiple 3D microscopy techniques, we demonstrate the impact of scattering on three tomographic phase microscopy (TPM) reconstruction methods. One of these methods assumes the sample to be weak-scattering, while the other two take multiple scattering into account. The study is performed at two wavelengths (visible and near-infrared), which serve as a scaling factor for the scattering phenomenon. We find that changing the wavelength from visible into near-infrared impacts the applicability of TPM reconstruction methods. As a result of reduced scattering in near-infrared region, the multiple-scattering-oriented techniques perform in fact worse than a method aimed for weak-scattering samples. This implies a necessity of selecting proper approach depending on sample’s scattering characteristics even in case of subtle changes in the object-light interaction.

Funder

Politechnika Warszawska

Fundacja na rzecz Nauki Polskiej

Cockrell School of Engineering, University of Texas at Austin

Chan Zuckerberg Initiative

Publisher

Springer Science and Business Media LLC

Subject

Multidisciplinary

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