Author:
Wang Xing,Kong Dehuang,van Dijk Gertjan,Meijer Harro A. J.
Abstract
AbstractThe doubly labelled water (DLW) method is widely used to determine energy expenditure. In this work, we demonstrate the addition of the third stable isotope, 17O, to turn it into triply labelled water (TLW), using the three isotopes measurement of optical spectrometry. We performed TLW (2H, 18O and17O) measurements for the analysis of the CO2 production (rCO2) of mice on different diets for the first time. Triply highly enriched water was injected into mice, and the isotope enrichments of the distilled blood samples of one initial and two finals were measured by an off-axis integrated cavity output spectroscopy instrument. We evaluated the impact of different calculation protocols and the values of evaporative water loss fraction. We found that the dilution space and turnover rates of 17O and 18O were equal for the same mice group, and that values of rCO2 calculated based on 18O–2H, or on 17O–2H agreed very well. This increases the reliability and redundancy of the measurements and it lowers the uncertainty in the calculated rCO2 to 3% when taking the average of two DLW methods. However, the TLW method overestimated the rCO2 compared to the indirect calorimetry measurements that we also performed, much more for the mice on a high-fat diet than for low-fat. We hypothesize an extra loss or exchange mechanism with a high fractionation for 2H to explain this difference.
Funder
Chinese Government Scholarship
Publisher
Springer Science and Business Media LLC
Reference50 articles.
1. Lifson, N., Gordon, G. B. & McClintock, R. Measurement of total carbon dioxide production by means of D2O18. J. Appl. Physiol. 7, 704–710. https://doi.org/10.1152/jappl.1955.7.6.704 (1955).
2. Westerterp, K. R. Doubly labelled water assessment of energy expenditure: Principle, practice, and promise. Eur. J. Appl. Physiol. 117, 1277–1285. https://doi.org/10.1007/s00421-017-3641-x (2017).
3. Black, A. E., Coward, W. A., Cole, T. J. & Prentice, A. M. Human energy expenditure in affluent societies: An analysis of 574 doubly-labelled water measurements. Eur. J. Clin. Nutr. 50, 72–92 (1996).
4. Speakman, J. R. Doubly Labelled Water: Theory and Practice (Chapman and Hall, 1997).
5. Visser, G. H., Boon, P. E. & Meijer, H. A. J. Validation of the doubly labeled water method in Japanese Quail Coturnix C. japonica chicks: Is there an effect of growth rate? J. Comp. Physiol. B Biochem. Syst. Environ. Physiol. 170, 365–372. https://doi.org/10.1007/s003600000112 (2000).
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献